PsdVTM Pseudovirus-based Drug Screening System
Businesses / Posted 4 months ago by Thomas Schmitt / 48 views
As a commonly used viral tool, pseudoviruses facilitate the study of high-risk and highly pathogenic enveloped viruses that require biosafety level (BSL)-3 or higher laboratories in a BSL-2 environment. Pseudoviruses are usually based on the genome of virus with low biological risk (e.g., murine leukemia virus, MLV and vesicular stomatitis virus, VSV) or modifed virus (human immunodefciency virus, HIV), in which the envelope protein genes required for infecting host cells are replaced by reporter genes that can be used for in vitro detection, such as GFP and luciferase genes. At the same time, plasmids or stably expressed cell lines are used to express the envelope protein of the high-risk virus to be studied. The core genome and envelope proteins derived from two different viruses are assembled in vitro to form a complete pseudovirus particle, which could be secreted into the cell culture supernatant. At this time, the supernatant is collected and can be used to infect the target cells. Thus, the pseudoviruses can simulate the process of live virus infection by using the envelope protein of highly infectious virus.
- Listing ID: 53447
- Country: USA